Biomarker validation is the process of confirming and verifying the accuracy, reliability, and reproducibility of a biomarker for its intended use in diagnosing, predicting, or monitoring a disease or health condition. This involves conducting rigorous studies to assess the sensitivity, specificity, and predictive value of the biomarker, as well as determining its performance in different populations or settings. Validation is essential to ensure that a biomarker is clinically useful and can be effectively implemented in healthcare practices. It typically involves multiple stages of testing, including analytical validation, clinical validation, and often validation in larger, diverse patient populations. Ultimately, validated biomarkers have the potential to improve patient outcomes, enable personalized medicine, and inform decision-making in healthcare.